[ASC-list] FW: AIBN Seminar: Associate Professor Gordon Xu, Associate AIBN Group Leader
jayne.keane at qm.qld.gov.au
Tue Oct 9 01:03:15 UTC 2012
AIBN SEMINAR SERIES 2012:
Associate Professor Gordon Xu, Associate AIBN Group Leader
Biomedical Applications of Layered Double Hydroxide Nanoparticles
Friday, 12 October 2012
Physiology Lecture Theatres (Building 63) - Lecture Theatre 360
Abstract: Layered double hydroxides (LDHs), also known as anionic
clays, find a high potential in nanomedicinal application as the
drug/gene delivery vehicle. These nanomaterials show a number of
advantages over the counter-part organic ones, such as easy preparation
in lab at low cost, precise control in particle size, low cytotoxicity,
good biocompatibility, and high endosomal escapability. We have
developed a robust way to control prepare LDH nanoaprticles (NPs) with
the particle size of 30-300 nm in a stable homogeneous suspension.
We have first found that LDH NPs can be quickly taken up by various cell
lines, in a clathrin-mediated dose-dependent and time-dependent
endocytosis pathway. More excitedly, we have for the first time
discovered that the rod-like LDH NPs are mostly located in the nucleus
while plate-like LDH NPs in the perinuclear area, which may imply a
strategy that can be used to target the subcellular compartments by
controlling the shape/size of delivery vehicles.
We have then found that LDH NPs can deliver supercoiled plasmid DNA (ca.
6.1k bps) into various cell lines with a transfection efficacy up to
70-80% cells in the optimized conditions. Moreover, LDH NPs are able to
siRNA into CHO cells to knockdown the expression of the target protein
with the efficiency compared with the commercial delivery vehicle. We
have shown that cultured neurons can more efficiently internalize
siRNA-LDH NPs than non-neuronal cells (eg. fibroblasts), and that
release of siRNA into the neuronal cytoplasm resulted in knockdown of
the axon guidance receptor DCC.
We have also found that LDH NPs are able to deliver anti-restenotic
drug, low molecular weight heparin (LMWH) to vascular smooth muscle
cells (SMCs). Intercalation of LMWH into LDH enables the release to
sustain for over 5 days. LMWH-LDH nanohybrids enhance the inhibition to
SMC proliferation and migration by ~60% compared with the control, e.g.
promoting the biological functions of LMWH on SMCs, which is actively
pursued for anti-restenosis treatment.
Very recently, we have found for the first time that LDH can be used as
a good antigen carrier for effective promotion of the antibody response.
The activity of LDH nanoparticles is even higher than the commonly used
adjuvant QuilA. More works are underway.
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